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. 2022 Jun 29;12:822760. doi: 10.3389/fonc.2022.822760

Figure 4.

Figure 4

HOXB5 promotes HCC progression by upregulating EGFR expression, and positively correlated with EGFR expression in human HCC tissues. (A) Expression of EGFR in HepG2-HOXB5 and Hep3B-shHOXB5, and their corresponding control cell lines as tested by Western bolt. Data are mean ± SD. **p < 0.01; (B) CCK8 and clonogenicity assays of proliferation capability of HCC cells. Data are mean ± SD. *p < 0.05; (C) Migration assay of HepG2-HOXB5 and Hep3B-shHOXB5, and their corresponding control cell lines as tested by transwell experiment. Data are mean ± SD. *p < 0.05, **p < 0.01. Scale bars, 100µm; (D) mRNA expression level of EGFR in 100 pairs of HCC tissues and adjacent non-tumor tissues was evaluated by qRT-PCR. Data are mean ± SD, n=100; (E) EGFR expression is positively corelated with HOXB5 expression; (F) Kaplan-Meier analysis showed that HCC patients with positive co-expression of EGFR and HOXB5 showed lowest OS and DFS times.