TABLE 3.
PCR primers and products for detection virulence determinants
| Gene and primer | Sequence (5′ to 3′) | Product size (bp) |
|---|---|---|
| agg | ||
| TE3 | AAGAAAAAGAAGTAGACCAAC | 1,553 |
| TE4 | AAACGGCAAGACAAGTAAATA | |
| gelE | ||
| TE9 | ACCCCGTATCATTGGTTT | 419 |
| TE10 | ACGCATTGCTTTTCCATC | |
| cylM | ||
| TE13 | CTGATGGAAAGAAGATAGTAT | 742 |
| TE14 | TGAGTTGGTCTGATTACATTT | |
| cylB | ||
| TE15 | ATTCCTACCTATGTTCTGTTA | 843 |
| TE16 | AATAAACTCTTCTTTTCCAAC | |
| cylA | ||
| TE17 | TGGATGATAGTGATAGGAAGT | 517 |
| TE18 | TCTACAGTAAATCTTTCGTCA | |
| esp | ||
| TE34 | TTGCTAATGCTAGTCCACGACC | 933 |
| TE36 | GCGTCAACACTTGCATTGCCGAA | |
| efaAfs | ||
| TE5 | GACAGACCCTCACGAATA | 705 |
| TE6 | AGTTCATCATGCTGTAGTA | |
| efaAfm | ||
| TE37 | AACAGATCCGCATGAATA | 735 |
| TE38 | CATTTCATCATCTGATAGTA | |
| cpd | ||
| TE51 | TGGTGGGTTATTTTTCAATTC | 782 |
| TE52 | TACGGCTCTGGCTTACTA | |
| cob | ||
| TE49 | AACATTCAGCAAACAAAGC | 1,405 |
| TE50 | TTGTCATAAAGAGTGGTCAT | |
| ccf | ||
| TE53 | GGGAATTGAGTAGTGAAGAAG | 543 |
| TE54 | AGCCGCTAAAATCGGTAAAAT | |
| cad, TE41 | TTATTTWSWTTAGTWTTAGCWGGW |