. 2001 Apr;67(4):1628–1635. doi: 10.1128/AEM.67.4.1628-1635.2001

TABLE 4.

Summary of PCR, Southern hybridization, and phenotypic screening results for panel A isolates

Strain	Species	Source(s)	Genotype	Relevant phenotype
Starters
F12	E. faecium	Cheese starter, dried milk	efaAfm⁺
F13	E. faecium	Cheese starter, dried milk	efaAfm⁺
F20	E. faecium	Commercial milk product starter	efaAfm⁺
F21	E. faecium	Commercial milk product starter	efaAfm⁺
F22	E. faecium	Spanish sausage starter	efaAfm⁺
F23	E. faecium	Spanish sausage starter (including homemade)	efaAfm⁺
F25	E. durans	Cheddar cheese starter
F27	E. faecalis	Spanish Manchego cheese starter	efaAfs⁺agg⁺cpd⁺cob⁺ccf⁺cad⁺
F41	E. faecalis	Commercial milk product starter	efaAfs⁺gelE⁺agg⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻
Food
F1	E. faecalis	Sour milk	efaAfs⁺gelE⁺agg⁺cylMBA⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻ Hyl⁺
F2	E. faecalis	Danish blue cheese	efaAfs⁺agg⁺cylMBA⁺cpd⁺cob⁺ccf⁺cad⁺	Hyl⁺
F3	E. faecalis	Danish blue cheese	efaAfs⁺agg⁺cylMBA⁺cpd⁺cob⁺ccf⁺cad⁺	Hyl⁺
F4	E. faecalis	Stilton cheese	efaAfs⁺gelE⁺agg⁺cylMBA⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻ Hyl⁺
F5	E. faecalis	Milk	efaAfs⁺gelE⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺
F6	E. faecalis	Dairy cheese	gelE⁺agg⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺
F7	E. faecalis	Raw milk	efaAfs⁺gelE⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺
F8	E. faecalis	Raw milk	efaAfs⁺gelE⁺agg⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺
F9	E. faecium	Cheese	efaAfm⁺ (efaAfs⁺)
F10	E. faecium	Cheese	efaAfm⁺
F11	E. faecium	Fermented-food waste	efaAfm⁺ (efaAfs⁺)
F14	E. faecium	Commercial milk	efaAfm⁺ (efaAfs⁺)
F15	E. faecium	Cheddar cheese	efaAfm⁺
F16	E. faecium	Cheddar cheese	efaAfm⁺ (efaAfs⁺)
F17	E. faecium	Canned ham	efaAfm⁺ (efaAfs⁺)
F19	E. faecium	Uncooked sausage	efaAfm⁺ (efaAfs⁺)
F24	E. durans	Cheddar cheese	efaAfm⁺ (efaAfs⁺)
F26	E. durans	Pasteurized milk	efaAfs⁺
F28	E. faecium	Slaughterhouse broiler
F29	E. faecium	Retail broiler
F31	E. faecium	Retail pork	efaAfm⁺ (efaAfs⁺)
F32	E. faecalis	Retail pork	efaAfm⁺efaAfs⁺gelE⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺
Medical
P1	E. faecalis	Endocarditis (efaAfs⁺ control)	efaAfs⁺gelE⁺agg⁺cpd⁺ cob⁺ ccf⁺ cad⁺	GelE⁺
P3	E. faecalis	Human mouth (gelE⁺ control)	efaAfs⁺gelE⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺
P4	E. faecalis	Endocarditis (cylMBA⁺agg⁺ control)	efaAfs⁺gelE⁺agg⁺ cylMBA⁺ cpd⁺ cob⁺ ccf⁺ cad⁺	GelE⁺ Hyl⁺
P11	E. faecium	Blood (Oxford) (esp⁺efaAfm⁺ control)	efaAfm⁺ (efaAfs⁺) esp⁺
P13	E. faecium	Pus (Yeovil)	efaAfm⁺ (efaAfs⁺)
P14	E. faecalis	Blood (Newcastle)	efaAfs⁺gelE⁺agg⁺cylMBA⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁺ > GelE⁻ Hyl⁺
P20	E. faecium	Perineum (Plymouth)	efaAfm⁺ (efaAfs⁺) gelE⁺esp⁺	GelE⁺ > GelE⁻
P21	E. faecalis	Wound swab (St. Helier hospital)	efaAfs⁺gelE⁺agg⁺cylMB⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻ Hyl⁻
P26	E. faecalis	Feces (Glasgow)	efaAfs⁺gelE⁺agg⁺cylMBA⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻ Hyl⁺
P31	E. faecalis	Urine (N.E. Lincs.)	efaAfs⁺gelE⁺agg⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻
P36	E. faecalis	Blood (Plymouth)	efaAfs⁺gelE⁺agg⁺cylMBA⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺	GelE⁻ Hyl⁻
P41	E. faecalis	Blood, endocarditis (Salisbury)	efaAfs⁺esp⁺cpd⁺cob⁺ccf⁺cad⁺
P46	E. faecium	Wound swab (Cardiff)	efaAfm⁺ (efaAfs⁺)
P51	E. faecium	Feces (Harrow)	efaAfm⁺ (efaAfs⁺) esp⁺
P56	E. faecium	Urine (Cheltenham)	efaAfm⁺ (efaAfs⁺) esp⁺
P61	E. faecium	Blood (Stafford)	efaAfm⁺ (efaAfs⁺) esp⁺
P66	E. faecium	Blood, endocarditis (Dyfed)	efaAfm⁺ (efaAfs⁺) esp⁺
P71	E. faecium	Environment (Bristol)	efaAfm⁺ (efaAfs⁺) esp⁺

GelE⁺ > GelE⁻ is loss of gelatinase activity. Hly is cytolysin activity. efaAfm⁺efaAfs⁺ indicate that a signal was obtained with efaA E. faecium- and E. faecalis-specific probes, respectively. Parentheses indicate that a weak signal was obtained under standard-stringency conditions. cpd⁺, cob⁺, ccf⁺, and cad⁺ indicate the presence of the corresponding pheromone-encoding genes.