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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Genesis. 2020 Sep 29;58(10-11):e23395. doi: 10.1002/dvg.23395

Fig.3. Loss of function of cul-4 affects the retinal determination (RD) gene network.

Fig.3

(A)The RD gene network in developing Drosophila eye includes a hierarchy of interaction(s) between Twin of Eyeless (Toy), Eyelsss (Ey), Eyes absent, (Eya), Sine oculis (So) and Dacshund (Dac). (B-G) Third instar eye-antennal imaginal disc stained for retinal determination markers and pan-neural marker Elav (red), which marks the photoreceptors. Note that the wild-type expression of (B) Ey (green, B’ split channel), (D) Eya (green, D’ split channel), and (F) Dac (green, F’ split channel) is significantly affected in (C, E, G) cul-4 mutant. Note that levels of RD genes remain unaffected in the antenna. The magnification of all eye-antennal imaginal disc is 20X. A total of five eye-antennal imaginal discs (n=5) for each genotype were analyzed for respective immunohistochemistry staining.