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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Genesis. 2020 Sep 29;58(10-11):e23395. doi: 10.1002/dvg.23395

Fig. 5. Loss of function of cul-4 promotes ectopic expression of negative regulators of retinal development.

Fig. 5

Secreted morphogen Wingless (Wg) is required for antenna and head development but is a negative regulator of MF progression as well as retinal development. Eye antennal imaginal discs are stained for pan-neural marker Elav (red). (A, A’) Wg (green) is expressed in dorso-lateral margins of the developing eye-antennal imaginal disc. (B, B’) Loss of function of cul-4 results in increase in the levels of Wg along these margins. (C, C’) Meis class protein Homothorax (Hth, green) is a negative regulator of the eye development and is expressed only in the undifferentiated head cuticle region and in the peripodial epithelial cells. (D, D’) Loss of function of cul-4 results in extension of Hth levels all the way into differentiated photoreceptor neuron region. The magnification of all eye-antennal imaginal disc is 20X. A total of five eye-antennal imaginal discs (n=5) for each genotype were analyzed for respective immunohistochemistry staining.