Fig. 5.

Ependymal cell channels are reduced with LV-proximal GBM. A Representative coronal immunohistochemistry images in vehicle (left), LV-distal GBM (middle), and LV-proximal GBM (right) groups of ependymal cell connexin-43 junctions showing loss of junctions in LV-proximal GBM (n = 5). White arrows indicate junctional loss. The lateral ventricle is indicated with a white asterisk. The choroid plexus is indicated as “CP”. Scale bar = 10 µm. B Quantification of number of Cx-43 + puncta. C Representative coronal immunohistochemistry images in vehicle (left), LV-distal GBM (middle), and LV-proximal GBM (right) groups of ependymal cell AQP4 channels showing loss of channels in LV-proximal GBM (n = 5). White arrows indicate channel loss. The lateral ventricle is indicated with a white asterisk. Scale bar = 10 µm. D Quantification of number of AQP4 + channels. The data are presented as mean ± standard error of the mean. Statistical test used was ANOVA with Tukey’s post-hoc correction. *p < 0.05, **p < 0.01. Non-significant interactions are not indicated