FIG. 4.
DNA content of C. oligotrophus. (A) DNA histogram for a stationary-phase culture with chromosome runout. The coefficients of variation for the one- to five-chromosome peaks ranged from 4.28 to 8.73%. (B) Linearity between DNA content determined from the standard curve and modal values for the fluorescence intensity from panel A. (C) Relationships between DNA content and dry mass and between DNA content and DNA concentration. Values were calculated by using a genome size of 3.5 fg cell−1 and a dry weight/wet weight ratio of 0.2; dry mass was determined by using forward light scatter intensity data (49).