Figure 1.

THP-1 cells were induced to functional TAMs

(A) Schematic diagram of macrophages induction. (B) The expression of CD86 and CD163 protein was measured by flow cytometry after macrophage induction. (C and D) The protein expression of Arg-1 and CD206 was detected by western blotting. The relative density ratio of each band was normalized to actin. (E) The concentration of IL-6, IL-10, and VEGFα protein was measured by ELISA assay. Data are presented as the mean ± SD (n = 3). ∗∗p < 0.01 versus M0. ^##p < 0.01 versus M1. (F) Cell viability of SKOV3 cells was measured by CCK-8 assay. SKOV3 cells were incubated with the specified conditioned medium (CM) for 5 days. Quantitative is relative to each group of 0 day, set to 100%. Data are presented as the mean ± SD (n = 3). ∗∗p < 0.01 versus control medium. ^##p < 0.01 versus M0 CM.