Figure 4.

Cardamonin decreased M2 polarization in TAMs

THP-1 cells were induced and treated with or without cardamonin (10 or 20 μM) and rapamycin (0.1 μM) for 24 h. (A–D) Surface markers CD86 and CD163 were analyzed by flow cytometry. The percentage of dyed cells for each group is shown as the bar graph. (E–G) Expression of the Arg-1 and CD206 protein was detected by western blotting. Relative protein expression was normalized to actin. Data are presented as the mean ± SD (n = 3). ∗∗p < 0.01 versus M0. ^#p < 0.05, ^##p < 0.01 versus TAM.