Figure 6.

Cardamonin inhibited tumors growth and decreased M2 macrophages in vivo

BALB/c nude mice were injected subcutaneously with SKOV3 cells or a mixture of SKOV3 cells and M2 macrophages. Then mice were treated with or without indicated drugs for 15 days (n = 6 per group). (A) Representative images of sacrificed mice. (B) Tumor weight. (C) Change of tumor volume during drug administration. Tumor size was calculated according to the formula: [length × (width)2]/2. (D) Representative images of hematoxylin and eosin (H&E) staining and immunohistochemistry for tumor histology analysis (scale bar, 200 μm). (E–G) The immunohistochemistry (IHC) index of Ki67(E), CD163 (F), and CD206 (G) in tumor tissue. Data are presented as the mean ± SD (n = 3). ∗∗p < 0.01 versus control group. ^#p < 0.05 versus control group of SKOV3 injection.