Fig. 3.

Enriched BT_0972 protein (BT_0972p) exhibits 3-KDSR activity. A: Enriched BT_0972p was analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue. B: Scheme for sphingolipid biosynthesis. Mass spectrometry ion chromatograms of (C) 3-ketosphingaine alkyne (3-KDS^alk) and (D) sphinganine alkyne (SA^alk) demonstrating that the purified BT_0972p successfully produced SA^alk. +PBS and +Mock are negative controls that represent vehicle and mock purification control respectively (n = 2 biological replicates per gene candidate and positive controls; representative traces are shown here.) 3-KDS^alk, 3-ketosphinganine alkyne; 3-KDSR, 3-ketosphinganine reductase; SA^alk, sphinganine alkyne.