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. 2022 Jul 1;16:100342. doi: 10.1016/j.mtbio.2022.100342

Fig. 7.

Fig. 7

Laponite activates PI3K/AKT signal transduction pathway-related proteins (p-PI3K, p-AKT) of BMSCs in hydrogel scaffolds to promote osteogenic differentiation in vitro. Expression levels of target proteins as determined by western blotting (a) and phosphorylation levels of PI3K, AKT and mTOR proteins were significantly higher in Hyd_Lap group (b) compared with Blank and Hyd groups. αp<0.05 vs Blank; βp<0.05 vs Hyd. (c, d) Expression levels of Runx2 and Col 1α in Hyd_Lap group, Blank and Hyd groups. Protein levels of Runx2 and Col 1α were significantly suppressed in Hyd_Lap ​+ ​LY294002 group after treatment with LY294002. αp<0.05 vs Blank; βp<0.05 vs Hyd; γp<0.05 vs Hyd_Lap. (e, f) Mineralization nodules were higher in Hyd_Lap than in Hyd group, and treatment of LY294002 reduced mineralization nodules. Scale bars: black 200 ​μm; red 100 ​μm αp<0.05 vs Hyd; βp<0.05 vs Hyd_Lap. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)