Fig. 2.

BDL is associated with hyperpolarisation of ΔΨm, which is restored by OP treatment.

(A) Representative images of TMRM fluorescence in acute brain slices from sham-operated, BDL, and BDL/OP rats. (B) Quantification bar-chart of ΔΨm in acute slices from sham-operated, BDL, and BDL/OP rats. (C) representative traces of dynamic changes of TMRM intensity from sham-operated (C1), BDL (C2), and BDL/OP (C3) rat brain slices upon the application of oligomycin (2 μg/ml), rotenone (1 μM) and FCCP (1 μM). Scale bar = 50 μm. Data are represented as mean ± SEM. ∗p <0.05, ∗∗p <0.001, ∗∗∗p <0.0001, two-tailed unpaired Student’s t test. ΔΨm, mitochondrial membrane potential; BDL, bile duct ligation; FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; OP, ornithine phenylacetate; TMRM, tetramethyl rhodamine, methyl ester.