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BLI kinetic experiment with SARS-CoV-2 S1-S2 and hACE2. The sensogram (A) was globally fitted with a 1:1 interaction model (B and C, black dashed lines) either with a cropped dissociation time of 200 s (B) or the full dissociation time of 600 s (C). (B) KD, 22 nM; ka, 1.1 E + 5 ± 7.5 E + 4 [1/Ms]; kd, 2.4 E-3 ± 4.7 E-3 [1/s]. R2, 0.98. (C) KD, 11 nM; ka, 1.7E + 5 ± 1.5 E + 5 [1/Ms], kd, 1.9 E-3 ± 4.7 E-3 [1/s]. R2, 0.96.
