TABLE 2.
Primer | Forward sequence (5′–3′) | Reverse sequence (5′–3′) |
---|---|---|
Prespliced IκBα | AGG GTG GCA ACA AGT GCT TA | AGC ACC CAA AGA CAC CAA CA |
Prespliced GAPDH | CAT CCA CAG TCT TCT GGG TGG | TTC ACT CCC CCT CCT TGT CTC |
Prespliced B2M | GGC GGA TGG AAC CCA GAT AC | CGG TGA AAT CCT CTG GCG TT |
Prespliced PGK1 | GGA AGA ATT GCT AGG GAC TGA | TCC AGC CTT CTT TGG CAG ATT |
rRNA 28S (60) | GGG CCG AAA CGA TCT CAA CC | GCC GGG CTT CTT ACC CAT T |
The forward sequence is that closer to the 5′ end of the chromosome in which the gene is located.