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. 2022 Jun 14;96(13):e00383-22. doi: 10.1128/jvi.00383-22

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This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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FIG 1 — Design and construction of S1-conjugated nanoparticles. Shown is a structural representation of SARS-CoV-2 spike protein. (A) The SARS-CoV-2 spike consists of two segments, S1 and S2 (light yellow), and the S1 segment includes NTD (light blue), RBD (dark green), and CTD (light green). (B) Structural representation and construction of S1-conjugated nanoparticle components. The S1 domain was conjugated to the nanoparticle ferritin, mi3, or I53-50 by the formation of a covalent bond between SpyTag and SpyCatcher. (C) SDS-PAGE of the S1 monomer and three S1-conjugated nanoparticles. The conjugated nanoparticles were resisted to the DTT reduction. (D) Size exclusion chromatography (SEC) of the S1 monomer, unconjugated nanoparticles, and S1-conjugated nanoparticles. After conjugation, the molecular weight of nanoparticles significantly increased. The normalized A₂₈₀ absorbance curve is presented.