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. 2022 Jun 14;96(13):e00383-22. doi: 10.1128/jvi.00383-22

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FIG 2 — Assembly efficiency and the storage stability of S1-conjugated nanoparticles. (A) SDS-PAGE assembling assays of S1-SpyTag and SpyCatcher-nanoparticle scaffolds at different mixing ratios. As the ratio of SpyCatcher-ferritin/mi3/I53-50A1.PT1 and S1 reached 1:8, the remnant unconjugated nanoparticle scaffolds were almost depleted, suggesting that the nanoparticles were fully conjugated with S1 at high superficial abundance. (B) SDS-PAGE assembling assays of S1-SpyTag and SpyCatcher-nanoparticle at different temperatures. No significant degradation or aggregation was observed among S1 monomer or S1 nanoparticles.