FIG 3.

FUBP3 can make interaction with the PEDV N protein. (A) Plasmids that encoded Flag-FUBP3 and HA-N were exposed to transfection into HEK 293T cells. Subsequently, a Co-IP assay was carried out by adopting anti-Flag-bound beads, and WB was conducted for analysis; ACTB was employed to be a sample loading control. (B) The cells cotransfected with Flag-FUBP3 and HA-N for 24 h were collected. RNase was used to incubate the lysate, and the relationship between FUBP3 and the N protein was analyzed through Co-IP assays. (C) Vero cells were mock-infected or infected with PEDV (MOI = 0.01) and were harvested 24 h after being infected. Co-IP assays were conducted using the anti-PEDV N protein antibody. (D) FUBP3 and GST-N expression was induced within the bacterial strain BL21(DE3), with the relationship between FUBP3 and the N protein being analyzed by performing a GST pulldown analysis. (E) N-mCherry and FUBP3-GFP were cotransfected into HeLa cells for a whole day. In addition, the cell nuclei were stained with DAPI, and the colocalization of FUBP3 and N was observed through confocal immunofluorescence microscopy; scale bars: 100 μm.