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. 2022 Jun 13;96(13):e00618-22. doi: 10.1128/jvi.00618-22

TABLE 1.

The sequences of the primers and siRNAs

Purpose Names Sequence (5′–3′)
Real-time PCR Primers PEDV N forward GAGGGTGTTTTCTGGGTTG
PEDV N reverse CGTGAAGTAGGAGGTGTGTTAG
pFUBP3 forward GCATAAACCAGCAGTCGGG
pFUBP3 reverse TGAAGGGGCTCTGTCCAAA
TCFL5 forward AGCAGGTTTGGATTAAAGTAGG
TCFL5 reverse AGGCTGGACTGCGAGGA
ACTB forward TCCCTGGAGAAGAGCTACGA
ACTB reverse
pGAPDH forward
pGAPDH reverse
hIFN-β forward
hIFN-β forward
AGCACTGTGTTGGCGTACAG
ATGGATGACGATATTGCTGCGCTC
TTCTCACGGTTGGCTTTGG
TCTTTCCATGAGCTACAACTTGCT
GCAGTATTCAAGCCTCCCATTC
ChIP assay ChIP primer forward CAGAGACGGGACGGCAGCCTCACGC
ChIP primer reverse GGACCCGTAGCCGGAAAGGGAAAGT
siRNA sequences si-FUBP3 sense
si-FUBP3 antisense
si-TCFL5 sense
si-TCFL5 antisense
si-MyD88 sense
si-MyD88 antisense
si-TRAF3 sense
si-TARF3 antisense
si-TRAF6 sense
si-TRAF6 antisense
NC sense
NC antisense
GCGUGAAGAUGGUCAUGAUTT
AUCAUGACCAUCUUCACGCTT
CGACAUCCGUCCGAAUUAATT
UUAAUUCGGACGGAUGUCGTT
GUACAAGGCAAUGAAGAAATT
UUUCUUCAUUGCCUUGUACTT
GGCCGUUUAAGCAGAAAGUTT
ACUUUCUGCUUAAACGGCCTT
GCGCUGUGCAAACUAUAUATT
UAUAUAGUUUGCACAGCGCTT
UUCUCCGAACGUGUCACGUTT
ACGUGACACGUUCGGAGAATT