TABLE 1.
Purpose | Names | Sequence (5′–3′) |
---|---|---|
Real-time PCR Primers | PEDV N forward | GAGGGTGTTTTCTGGGTTG |
PEDV N reverse | CGTGAAGTAGGAGGTGTGTTAG | |
pFUBP3 forward | GCATAAACCAGCAGTCGGG | |
pFUBP3 reverse | TGAAGGGGCTCTGTCCAAA | |
TCFL5 forward | AGCAGGTTTGGATTAAAGTAGG | |
TCFL5 reverse | AGGCTGGACTGCGAGGA | |
ACTB forward | TCCCTGGAGAAGAGCTACGA | |
ACTB reverse pGAPDH forward pGAPDH reverse hIFN-β forward hIFN-β forward |
AGCACTGTGTTGGCGTACAG ATGGATGACGATATTGCTGCGCTC TTCTCACGGTTGGCTTTGG TCTTTCCATGAGCTACAACTTGCT GCAGTATTCAAGCCTCCCATTC |
|
ChIP assay | ChIP primer forward | CAGAGACGGGACGGCAGCCTCACGC |
ChIP primer reverse | GGACCCGTAGCCGGAAAGGGAAAGT | |
siRNA sequences | si-FUBP3 sense si-FUBP3 antisense si-TCFL5 sense si-TCFL5 antisense si-MyD88 sense si-MyD88 antisense si-TRAF3 sense si-TARF3 antisense si-TRAF6 sense si-TRAF6 antisense NC sense NC antisense |
GCGUGAAGAUGGUCAUGAUTT
AUCAUGACCAUCUUCACGCTT CGACAUCCGUCCGAAUUAATT UUAAUUCGGACGGAUGUCGTT GUACAAGGCAAUGAAGAAATT UUUCUUCAUUGCCUUGUACTT GGCCGUUUAAGCAGAAAGUTT ACUUUCUGCUUAAACGGCCTT GCGCUGUGCAAACUAUAUATT UAUAUAGUUUGCACAGCGCTT UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |