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. 2022 Jul 6;172(4):205–216. doi: 10.1093/jb/mvac054

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© The Author(s) 2022. Published by Oxford University Press on behalf of the Japanese Biochemical Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Effects of pull-down captured proteins on S1-ACE2 binding. ELISA was performed to determine the effects of human neutrophil elastase (a), neutrophil defensin-1 (b) and r-histone H2A (c) at the indicated concentrations. In neutrophil elastase experiments, the effects were examined in the presence (Inhibitor, 100 μM) and absence (Vehicle) of its inhibitor, elastatinal. The rate of inhibition was the average ± SD (error bar) of three determinations. The experiment was conducted twice. d. ct-histone was also inhibited S1-ACE2 binding.