Table 1.
Frequently reported B-cell differentiation stimuli for human naive and/or memory B cells in literature.
| Stimuli | Target(s) | Concentrations | Reference |
|---|---|---|---|
| Anti-IgM Anti-IgG/IgM F(ab′)2 Anti-IgG/IgA/IgM F(ab′)2 |
BCR | 2, 5, 10 µg/ml | (5, 16–22) |
| BAFF | BAFF-R, BMCA, TACI | 75, 100 ng/ml | (19, 23) |
| αCD40, CD40L | CD40 | 50, 500 ng/ml 1, 5 µg/ml |
(5, 16, 18, 21, 22, 24–26) |
| CD40L expressing L cells 3T3-CD40L fibroblasts |
CD40 | Various ratios of B cell:feeder cell | (3, 17, 19, 20) |
| CpG-ODN 2006 | TLR-9 | 1.0, 2.5, 3.2, 6.0, 10 µg/ml 0.35, 1.0 µM |
(16, 18, 19, 21–26) |
| IFNα | IFNAR | 100, 500 U/ml | (20, 24) |
| IL-2 | IL-2R | 20, 50, 100 U/ml 25, 50 ng/ml |
(3, 5, 20–22, 24, 25) |
| IL-4 | IL-4R | 10, 25, 50, 100, 200 ng/ml | (3, 5, 16, 17, 19, 21, 24, 26) |
| IL-6 | IL-6R | 10, 50 ng/ml | (20, 24) |
| IL-10 | IL-10R | 25, 50, 200 ng/ml | (5, 19, 21, 24, 26) |
| IL-15 | IL-15R | 10 ng/ml | (19, 24) |
| IL-21 | IL-21R | 2, 20, 50, 100 ng/ml | (5, 17–20, 22, 23, 25, 26) |
The concentrations used in this study are depicted in bold. Abbreviations: BAFF, B-cell-activating factor; BCMA, B-cell maturation antigen; IFN, interferon; IL, interleukin; ODN, oligodeoxynucleotide; TACI (TNFRSF13B), transmembrane activator and CAML interactor; TLR, toll-like receptor.