TABLE 1.
16S rDNA sequences identified in anoxic water column of the Cariaco Basin in three clone libraries
| Phylogenetic affiliation | Representative clone (accession no.) | No. of clones in library
|
Closest relative
|
||||
|---|---|---|---|---|---|---|---|
| 320 m | 500 m | 1,310 m | Taxon | Accession no. | % Identity | ||
| Archaea | |||||||
| Crenarchaeota | Car132fc (AF224859) | 2 | Archaeon C20 | U71114 | 98.9 | ||
| Euryarchaeota | Car127c (AF224855) | 1 | Methanospirillum hungatei | M60880 | 74.5 | ||
| Car106b (AF224779) | 1 | Methanospirillum hungatei | M60880 | 74.9 | |||
| Car42fc (AF224831)a | 3 | Thermoplasma acidophilum | M38637 | 74.2 | |||
| Car68fc (AF224866) | 1 | Thermoplasma acidophilum | M38637 | 75.3 | |||
| Car154fc (AF224869) | 1 | Thermoplasma acidophilum | M38637 | 72.4 | |||
| Bacteria | |||||||
| Candidate division OP8 | Car60fc (AF224839) | 1 | OPB95 | AF027060 | 90.3 | ||
| Candidate division OP3 | Car11fa (AF224797) | 1 | OPB2 | AF027088 | 80.1 | ||
| Car136b (AF224787) | 2 | BD3-9 | AB015551 | 80.9 | |||
| Car30c (AF224825) | 1 | Koll 11 | AJ224540 | 82.6 | |||
| Car40rc (AF224830) | 1 | Geobacter metallireducens | L07834 | 81.2 | |||
| Car119rc (AF224848) | 1 | BD4-9 | AB015559 | 79.2 | |||
| Car121c (AF224850) | 1 | Methylobacterium sp. | D87985 | 79.1 | |||
| Car124rc (AF224853) | 1 | Clostridrium thermocellum | L09173 | 79.4 | |||
| Planctomycetes | Car65rc (AF224864) | 1 | BD2-3 | AB015533 | 85.9 | ||
| Car68rc (AF224865) | 1 | BD2-3 | AB015533 | 85.6 | |||
| Car122fc (AF224851) | 1 | BD2-3 | AB015533 | 85.2 | |||
| Fibrobacteria | Car104fb (AF224872)ab | 6 | SAR406 | U34043 | 89.4 | ||
| Car97fb (AF224822)b | 1 | SAR406 | U34043 | 86.1 | |||
| Car137rb (AF224788) | 1 | SAR406 | U34043 | 90.5 | |||
| Car53c (AF224775) | 2 | 1 | OCS307 | U41450 | 87.6 | ||
| Car129rc (AF224857)a | 2 | 2 | Clone pG-13 | AF001509 | 82.1 | ||
| Flexibacter-Cytophaga-Bacteroides | Car44rc (AF224833) | 1 | Bacteroides ovatus | X83952 | 92.2 | ||
| Car56rb (AF224809) | 1 | 1 | Sphingobacterium heparinum | M11657 | 98.5 | ||
| Car82fb (AF224811) | 1 | Flavobacterium balustinum | D14016 | 92.1 | |||
| Car114fc (AF224844) | 1 | Flavobacterium balustinum | D14016 | 99.2 | |||
| Car116rc (AF224845) | 1 | Acinetobacter sp. | Y15852 | 73 | |||
| Verrucomicrobia | Car98rb (AF224823) | 1 | Clone WCHB1-41 | AF050560 | 86.9 | ||
| Proteobacteria | |||||||
| Nonaffiliated proteobacteria | Car731c (AF224870) | 2 | A51p | X91474 | 88.2 | ||
| Car63a (AF224801) | 1 | Clone MUG16 | AB011308 | 73.2 | |||
| Car33fb (AF224808) | 2 | Lucina floridana symbiont | L25707 | 86.8 | |||
| β-Proteobacteria | Car117fc (AF224846) | 1 | Alcaligenes faecalis | AJ277669 | 97.5 | ||
| γ-Proteobacteria | Car16fa (AF224800) | 1 | Thiomicrospira sp. | L40811 | 86.7 | ||
| Car36rc (AF224827)a | 2 | Acyrthosiphon pisum symbiont | AB033779 | 93.0 | |||
| Car128fc (AF224856)c–e | 4 | 1 | Clone UN106 | D63592 | 99.4 | ||
| Car91fb (AF224817)c | 1 | Clone UN106 | D63592 | 99.7 | |||
| Car141fb (AF224791)d | 1 | Clone UN106 | D63592 | 99.3 | |||
| Car149fc (AF224862)e | 1 | Clone UN106 | D63592 | 99.7 | |||
| Car39fc (AF224829) | 1 | Citrobacter farmeri | AF025371 | 93.4 | |||
| Car70c (AF224867)a | 3 | 11 | PVB18 | U15114 | 99.1 | ||
| Car43fc (AF224832) | 1 | PVB18 | U15114 | 95.6 | |||
| Car140fb (AF224790) | 2 | PVB18 | U15114 | 95.5 | |||
| Car92rb (AF224818) | 1 | 1 | PVB18 | U15114 | 96.5 | ||
| Car123rc (AF224852) | 1 | Photobacterium phosphoreum | D25310 | 95.9 | |||
| δ-Proteobacteria | Car45fc (AF224834) | 1 | 2 | Clone SB-30 | AF029048 | 96.2 | |
| Car102rb (AF224777) | 1 | Clone SB-30 | AF029048 | 95.4 | |||
| Car148rc (AF224861) | 1 | Clone SB-30 | AF029048 | 88.4 | |||
| Car113fc (AF224843) | 1 | Clone SB-30 | AF029048 | 94.9 | |||
| Car99rb (AF224824) | 1 | Clone SB-30 | AF029048 | 91.4 | |||
| Car81fb (AF224810) | 1 | Clone A48u | X91471 | 90.0 | |||
| Car86rb (AF224813) | 1 | Clone NKB19 | AB013271 | 81.3 | |||
| Car93fb (AF224819) | 1 | Clone BD7-15 | AB015588 | 93.4 | |||
| Car95fb (AF224821) | 1 | Clone SB-21 | AF029045 | 93.9 | |||
| Car7ra (AF224796) | 1 | SAR276 | U65915 | 88.9 | |||
| Car150fc (AF224868) | 1 | RFLP25 | AF058007 | 95.7 | |||
| Car103rb (AF224871) | 2 | Bdellovibrio stolpii | AJ288899 | 90.5 | |||
| Car147fc (AF224860) | 1 | Strain S16 | Z69320 | 92.8 | |||
| Car37fc (AF224828) | 2 | Desulfobacter postgatei | M26633 | 83.6 | |||
| ɛ-Proteobacteria | Car153a (AF224805)a | 51 | Clone NKB12 | AB013264 | 94.6 | ||
| CarB13fa (AF224804) | 1 | Clone NKB12 | AB013264 | 96.7 | |||
| Car29rb (AF224806) | 3 | Clone NKB12 | AB013264 | 95.8 | |||
| Car55fb (AF224807) | 6 | Clone NKB12 | AB013264 | 95.6 | |||
| Car59rc (AF224838) | 4 | Clone NKB12 | AB013264 | 95.4 | |||
| Car125fc (AF224854) | 1 | Clone NKB12 | AB013264 | 95.6 | |||
| Car13a (AF224799) | 1 | Clone BD7-6 | AB013264 | 94.2 | |||
| Car2a (AF224795) | 1 | “Thiomicrospira denitrificans” | L40808 | 94.2 | |||
| Car107fb (AF224780) | 1 | “Thiomicrospira denitrificans” | L40808 | 91.7 | |||
| Car12fa (AF224798) | 2 | Arcobacter nitrofigilis | L14627 | 93.9 | |||
| Chimeras | 1 | 5 | 6 | ||||
| Total | 61 | 56 | 65 | ||||
a
Representative of a group of closely related sequences (>99% identity).
b
Car104fb and Car97fb exhibit 98.7% identity.
c
Car128fc and Car91fb exhibit 98.2% identity.
d
Car128fc and Car141fb exhibit 98.8% identity.
e
Car128fc and Car149fc exhibit 98.3% identity.