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Occludin, claudin4 and ZO-1 expression were up-regulated in senescent IEC6 cells by uridine treatment. The total protein was extracted. Each sample (30 μg) was subjected to SDS-PAGE, and then transferred to the PVDF membrane. After three washes with TBST, the membranes were incubated with the primary antibody at 4°C overnight. The cell samples were washed and incubated with the secondary antibody for 1 h at RT. (n = 5). The asterisk indicates a significant difference (p < 0.05).
