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. 2022 Jul 11;11(1):2094583. doi: 10.1080/2162402X.2022.2094583

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Immunofluorescence of tumor specimens from Mlh1^−/− and Msh2^{loxP/loxP;TgTg(Vil}¹^−cre) mice. Residual tumor slides were fixed, stained and embedded. Confocal laser scanning microscopy was done on a Zeiss Elyra 7 microscope. The infiltration pattern of T cells, regulatory and tumor-associated macrophages differed between individual treatment groups. In most cases, the differences reached statistical significance. Upper panel: representative images of tumor slides; lower panel: quantitative analysis of tumor-infiltrating immune cells. (a, d) Given is the number of infiltrating CD3⁺CD4⁺ T helper cells, CD3⁺CD8⁺ cytotoxic T cells and IRF5⁺ macrophages counted in 2–3 HPFs/slide with n = 3–10 mice/group. (b, c) The infiltration pattern was semi-quantitatively analyzed using a scoring system. 0 = no; 1 = mild; 2 = moderate; 3 = strong. Each symbol represents one case. *p < .05; **p < .01, Two-way ANOVA (Tukey’s multiple comparisons test).

Figure 5. — Immunofluorescence of tumor specimens from Mlh1^−/− and Msh2^{loxP/loxP;TgTg(Vil}¹^−cre) mice. Residual tumor slides were fixed, stained and embedded. Confocal laser scanning microscopy was done on a Zeiss Elyra 7 microscope. The infiltration pattern of T cells, regulatory and tumor-associated macrophages differed between individual treatment groups. In most cases, the differences reached statistical significance. Upper panel: representative images of tumor slides; lower panel: quantitative analysis of tumor-infiltrating immune cells. (a, d) Given is the number of infiltrating CD3⁺CD4⁺ T helper cells, CD3⁺CD8⁺ cytotoxic T cells and IRF5⁺ macrophages counted in 2–3 HPFs/slide with n = 3–10 mice/group. (b, c) The infiltration pattern was semi-quantitatively analyzed using a scoring system. 0 = no; 1 = mild; 2 = moderate; 3 = strong. Each symbol represents one case. *p < .05; **p < .01, Two-way ANOVA (Tukey’s multiple comparisons test).