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THM2 is dispensable for ciliogenesis but localizes to the primary cilium. a Western blot for THM2 on cellular extracts of 293T EV (control) and THM2 kd clonal lines. b Immunostaining for Arl13B (green) in 293T EV and THM2 kd (clone 2–1) cells. Scale bar—5 μm. c Quantification of cilia lengths. Each data point represents an individual cilium. Graphs represent mean ± SD. d Immunostaining for THM2 (green) and acetylated α-tubulin (red) in RPE cells. Scale bar—10 μm
