Fig. 6.
Thm2−/−; Thm1aln/+ tibia have less dense bone. a Radiographs of P14 skeleton and b vertebra. Scale bars—1 cm and 500 μm, respectively. c Caudal vertebrae lengths by number. d Caudal vertebrae/Crown-to-Rump (C-to-R) length ratios. Graphs represent mean ± SD of n = 3 ctrl and n = 3 triple allele mutant mice. Statistical significance was determined using two-tailed, unpaired t-test. *p < 0.05; **p < 0.01 e Radiographs of hindlimbs and f microCT imaging of tibias. Scale bars—5 mm and 1 mm, respectively. g Cortical bone mineral density (BMD), thickness and area at mid-diaphysis and h at the proximal end of tibia. i Cortical bone area/total bone area (fraction) at the proximal end of tibia. j Trabecular BMD at proximal end of tibia. Graphs represent mean ± SD of n = 3 ctrl and n = 3 triple allele mutant mice. Statistical significance was determined using a two-tailed, unpaired t test. *p < 0.05; **p < 0.005