Fig. 7.

Thm2^−/−; Thm1^aln/+ mice have altered tibial growth plates. a Haematoxylin and eosin (H&E) and b Safranin O and fast green staining of growth plates at P14. Scale bars—500 μm and 200 μm, upper and lower panels. PZ - proliferation zone, HZ - hypertropic zone c Quantification of % PZ/growth plate and % HZ/growth plate from H&E images. Error bars represent SD of n = 3 ctrl and n = 3 triple allele mutant growth plates. Statistical significance was determined using two-tailed, unpaired t test. **p ≤ 0.005