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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Radiat Res. 2022 Jul 1;198(1):89–105. doi: 10.1667/RADE-21-00224.1

Figure 4: LR-IL-22 gavage and preserves numbers of lgr5+ intestinal crypt stem cells following 6 Gy x 4 WAI.

Figure 4:

Figure 4:

Lgr5+ GFP+ mice (n = 13) were randomly divided into two groups: 1) 6 Gy x 4 WAI plus LR; 2) 6 Gy x 4 WAI plus LR-IL-22. Mice received either LR or LR-IL-22 intraorally (gavage) 24 hours prior to the initial fraction and 3 hours prior to each subsequent fraction (total of 4 doses, each equal to 109 copies of bacteria in 100 μL of saline). Mice were then sacrificed at day 0 prior to irradiation (n = 2), and day 4 (n = 5) and day 23 (n = 6) following last fraction of WAI for intestinal crypt cell analysis. (A) Total intestinal crypt cell quantification per section. (B) Percentage of Lgr5+ crypt cells per section. (C) Quantification of Lgr5+ crypt cells per section. (D) GFP+ intestinal crypt cells at ×40 magnification. (E) GFP+ intestinal crypt cells at ×200 magnification (*** = p < 0.05). (F) Lgr5+GFP+ stem cell numbers in irradiated ileum at days 0, 4, 23 for LR-IL-22 compared to LR (x 40 magnification). There were 5 mice per group per time point with 1000 cells scored in each of 30 microscopic sections per mouse.