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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Gastroenterology. 2022 Feb 22;162(7):2018–2031. doi: 10.1053/j.gastro.2022.02.024

Figure 3.

Figure 3.

Hypoxic CAFs promote macrophage migration in a HIF2-dependent paracrine fashion. (A) CM was collected from hypoxic CAFs treated with vehicle (veh) or PT2399. This CM was subsequently placed in the bottom chamber of a transwell as a chemoattractant for macrophage migration. (B) Representative bright-field images (10×; left) and quantification of macrophage migration relative to macrophages treated with CM from hypoxic veh-treated CAFs (right) of the transwell assay depicted in (A) (n = 8/group pooled from 3 independent experiments). (C) Five percent FBS/DMEM with veh or PT2399 was used as chemoattractant for macrophage migration. (D) Quantification of macrophage migration relative to macrophages treated with veh of the transwell assay depicted in (C). All error bars represent mean ± SEM; P, by 1-way analysis of variance; ns, not significant. See also Supplementary Figure 7.