Table 2.
Multiplexed immunofluorescence staining procedure
| Verify Tumor with Surgical Pathologist (serial H&E) | ||||
|---|---|---|---|---|
| Cy2 | Cy3 | Cy5 | Cy7 | |
| Round 1 | PRG2 | CD31 | CD141 | CD103 |
| Scan to Verify Quenched Slide (1) | ||||
| Round 2 | SMAα | MPO | LOX-1 | |
| Round 3 | CD62L | CD56 | CD8 | Granzyme B |
| Scan to Verify Quenched Slide (2) | ||||
| Round 4 | FOXP3 | CD163 | CD4 | |
| Round 5 | MUC1 | Tryptase | EPCAM | Ki67 |
| Scan to Verify Quenched Slide (3) | ||||
| Round 6 | CCR3 | CD3 | CD19 | CD11b |
| Round 7 | GFAP | IL10 | CXCR3 | CD45RA |
| Scan to Verify Quenched Slide (4) | ||||
| Round 8 | Vimentin | PD1 | FAP | |
| Round 9 | PDL1 | iNOS | ||
| Post-MxIF H&E Staining | ||||
| Verify Tumor/Stroma Areas with Surgical Pathologist | ||||