Bright-field micrographs of in vitro produced Staphylococcus aureus infected clots following 30-min treatment with a) plasma alone, b) plasma, rt-PA (thrombolytic), and oxacillin (antibiotic), and c) plasma, rt-PA, oxacillin, ultrasound, and Definity® (microbubble). The black arrow in image a) indicates the biofilm (beige). The thick black line, seen in all images and denoted by a white arrow in image a), is the suture to which the respective infected clots were adhered. Ultrasound parameters were 0.12 MHz and 0.44 MPa peak-to-peak pressure, intermittent (50 seconds on, 30 seconds off) continuous wave for 30 minutes (reprinted (adapted) with permission from Lattwein et al. (2017)).