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. 2022 Jun 29;10:893468. doi: 10.3389/fcell.2022.893468

FIGURE 4.

FIGURE 4

Crimson-CAAX labels the cytoplasmic membrane well in neurons. (A) Brightness comparison of RFP-CAAX fusions in rat hippocampal neurons 3 days post-transfection at 12 DIV. Representative confocal images of transfected neurons acquired and displayed with identical settings are shown. Scale bar = 20 µm. (B) RFP-CAAX fusions visualized with confocal microscopy 3 days post-transfection at 12 DIV, with image brightness adjusted to similar levels for display. Cell bodies are enlarged in the insets within the upper panels. Dendritic segments indicated by the dashed rectangle are enlarged in the lower panels. Scale bar = 20 µm (upper panels) or 5 µm (lower panels). (C) Quantification of brightness of RFP-CAAX constructs 3 days post-transfection at 12 DIV. Error bars = SEM. Overall p < 0.001 by one-factor ANOVA. (D) Quantification of the percentage of healthy neurons among total transfected neurons 6 days post-transfection at 15 DIV. Crimson-CAAX-expressing neurons demonstrated the highest viability. Error bars = SEM. Overall p < 0.0001 by one-factor ANOVA.