FIGURE 5.

Membrane-bound Crimson improves the detection of small processes. (A,B) Visualization of cultured rat hippocampal neurons with an epifluorescent microscope (A) and a confocal microscope (B). Neurons were co-transfected with cytosolic mTurquoise2 and Crimson-CAAX at 9 DIV and imaged at 14 DIV. Lower panels show dendrites from the same culture at higher magnification. Arrows indicate thin spines and filopodia visible with Crimson-CAAX but not with cytosolic mTurquoise2. Scale bars = 10 µm (upper panels) and 5 µm (lower panels). (C) Visualization of zebrafish trigeminal ganglion by two-photon microscopy in vivo. Zebrafish was injected with DNA at one-cell stage and imaged at 7-day post-fertilization. Scale bar = 20 µm.