Figure 4.

(A) In vitro cytotoxicity on colon cancer cells after 30 minutes of hyperthermia exposure (without dehydration) at 45°C and 48°C. Control cells were only exposed to the physiological temperature of 37°C. (B) In vitro cytotoxicity on colon cancer cells after 30 minutes of hyperthermia exposure and dehydration at 37°, 45°C and 48°C. (C) Cytotoxicity compared to control cells. Levels of cytotoxicity were measured at different exposure times to dehydration and hyperthermia (5, 10, 20, 25 and 30 minutes). Significance levels: #=p>0.05, *=p<0.05, **= p<0.001.