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. 2022 Jun 24;12:928484. doi: 10.3389/fonc.2022.928484

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Copyright © 2022 Austin, Huang, Wang, Cohen, Heilman, Maverakis, Michl and Jagdeo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RL increases the expression of immunocyte markers. (A) Quantification of CD103 (n=8), (B) CD68 (n=5 for 1280 J/cm², n=4 for 1920 J/cm², n=8 for 2560 J/cm²), (C) CD3 (n=4), and (D) CD4 (n=8) staining in the peritumoral dermis or skin from 1280, 1920, and 2560 J/cm² RL-treated and control C57BL/6 mice injected with B16F10 cells (two-tailed T-Test, p<0.05). (E) Representative IHC for CD103, CD68, CD3, and CD4 positive staining peritumoral dermis and skin. The staining intensity of each marker was indexed to the total skin or dermal area for each section. Spleen positive control tissue is provided. Staining was quantified using Indica HALO software and compared to total skin or dermal area. * denotes p<0.05, *** denotes p<0.001, ns denotes not significant.