Fig. 3. The function of Sen1 in RNAPIII transcription termination does not rely on Sen1 interaction with its partners Nrd1 and Nab3 or with the replisome.

(A) Metagene analysis of the RNAPIII distribution around tRNA genes as in Fig. 2 in a WT or in a Nrd1 AID strain in the absence or in the presence of IAA. Additional experiments validating the efficiency of Nrd1 depletion on well-characterized NNS target RNAs are included in fig. S2. (B and C) Individual examples of tRNA genes that exhibit clear termination defects in sen1-3 (see Fig. 2, B and C) but not under Nrd1-depleted conditions. (D) Metagene analysis as in Fig. 2A but in cells blocked in the G₁ phase of the cell cycle. (E and F) Individual examples of tRNA genes that display termination defects in the sen1-3 mutant in cells blocked in G₁ and in asynchronous cells (compare with Fig. 2, B and C).