. Author manuscript; available in PMC: 2022 Jul 13.

Published in final edited form as: J Vis Exp. 2021 Sep 21;(175):10.3791/62480. doi: 10.3791/62480

Table 1.

Overview of current protocols for differentiation of iPSCs to microglial cells.

Protocol	Supplements used	Sorting stage	Length of Differentiation	Protein markers

Abud et al. ⁴⁵	FGF, BMP4, Activin A, LiCl, FGF, VEGF, TPO, SCF, IL-3, IL-6, M-CSF, IL-34, TGFβ1, insulin, CD200, CX3CL1	At day 10, isolate CD34+ cells	38 days	CD45 CX3CR1 ITGB5 MERTK PROS1 TGFβR1 P2RY12 TREM2
Douvaras et al. ⁴⁹	BMP4, FGF, SCF, VEGF, IL-3, TPO, M-CSF, Flt-3L, GM-CSF, IL-34	At day 25, for CD14+/ CX3CR1+ cells	40 days	CD11b CD11c CX3CR1 IBA1 P2RY12 TMEM119
Haensler et al. ⁴⁸	BMP4, SCF, VEGF, M-CSF, IL-3, GM-CSF	At day 21, for CD11b+ / CD45+ cells	42 days	CD11b CD14 CD45 IBA1 MERTK