Table 2.

Overview of media used for microglial differentiation, listed with the concentration of cytokines used.

Day	Medium	Cytokines	Concentration
0–3	Stem Cell Medium	BMP-4	80ng/mL
4–5	Hematopoietic Medium	FGF SCF VEGF	25ng/mL 100ng/mL 80ng/mL
6–13	Hematopoietic Medium	SCF IL-3 TPO M-SCF Flt3-Ligand	50ng/mL 50ng/mL 5ng/mL 50ng/mL 50ng/mL
14–25+	Hematopoietic Medium	M-SCF Flt3-Ligand GM-SCF	50ng/mL 50ng/mL 50ng/mL
Adherent	RPMI 1640	GM-SCF IL-34	25ng/mL 100ng/mL