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. 2022 Mar 17;13(3):7367–7379. doi: 10.1080/21655979.2022.2040827

Figure 5.

Figure 5.

miR-940 directly bound to the 3’-UTR of ZDHHC19 and reversely regulated ZDHHC19 expression. (a) Bioinformatics analysis showed the miR-940 potential binding site located on the 3’-UTR of ZDHHC19. (b) The relative luciferase analysis based on a dual – luciferase reporter assay. (c) The expression status of miR-940 in OS cell lines (U2OS, MG63 and 143B) and the normal human bone cells (HFOB and HOBC). (d) The mRNA expression level of ZDHHC19 in OS cells transfected with inhibitor NC or miR-940 inhibitor. (e) The mRNA expression level of ZDHHC19 in OS cells transfected with mimics NC or miR-940 mimics. (f) The protein expression level of ZDHHC19 in OS cells transfected with miR-940 inhibitor or miR-940 inhibitor. All data are presented as the mean ± standard deviation of three independent experiments. *P < 0.05; **P < 0.01.