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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Nat Struct Mol Biol. 2021 Aug 11;28(8):662–670. doi: 10.1038/s41594-021-00633-2

Extended Data Fig. 5 |. FLCN-10 peptide is able to effectively inhibit dimeric but not tetrameric LDHA.

Extended Data Fig. 5 |

a–d) Immunoprecipitation of transiently expressed short segments of FLCN-FLAG protein implies the critical interacting region with LDHA. e) Ka of LDHA with FLCN-1 peptide (See Fig. 2c), measured by fluorescence polarization anisotropy (n=3). f) Ka plotted against LDHA activity in the presence of peptides. FLCN-1 peptide (blue) and FLCN-10 peptide (red) are highlighted. g) LDHA activity at 10 ng/μl (predominantly dimeric) and 500 ng/μl (predominantly tetrameric) in the presence of increasing amounts of recombinant FLCN protein or FLCN-10 peptide (n=3). Data shown as mean ± s.d. h) Data from Extended Data Fig. 5g presented as percent activity. Unpaired Student’s t-test, n=3. Data shown as mean ± s.d.