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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Nat Struct Mol Biol. 2021 Aug 11;28(8):662–670. doi: 10.1038/s41594-021-00633-2

Extended Data Fig. 7 |. inhibitory function of FLCN toward LDHA and its physiological outcome.

Extended Data Fig. 7 |

a) Western blot confirming transfection of FLCN-WT-FLAG and FLCN-M222A-FLAG or b) FLCN-WT-FLAG and FLCN-F226A-FLAG in UOK257 cells in Fig. 3d. c) Western blot confirming transfection of FLCN-WT-FLAG, FLCN-M222A-FLAG, or FLCN-F226A-FLAG in Fig. 3f. d) Representative images of UOK257 colonies in soft agar from Fig. 3f. Scale bar = 100 μM. e) LDHA activity in UOK257 cells transfected with EV, WT FLCN, FLCN-F118D-FLAG and FLCN-R164A-FLAG. Unpaired Student’s t-test, n=3. Data shown as mean ± s.d. *P < 0.05, **P < 0.005, ***P < 0.001.