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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Nat Struct Mol Biol. 2021 Aug 11;28(8):662–670. doi: 10.1038/s41594-021-00633-2

Extended Data Fig. 9 |. FLCN-10 peptide inhibits LDHA activity and induces cell death in ccRCC cell lines.

Extended Data Fig. 9 |

a) Caki-1 cells treated for 2 h with FLCN-10 or FLCN-13 were blotted using anti-cleaved caspase-3 and anti-pTyr10-LDHA. b) LDHA activity in HEK293 cells following 2 h treatment with FLCN-10 peptide. c) d) Flow cytometric assessment of cell death in renal cell lines following 1 μM FLCN-10 for 1 or 2 h (Extended Data Fig. 9c) or 0.1 μM or 1 μM FLCN-10 treatment for 2 h (Extended Data Fig. 9d) as determined by Annexin V/Propidium iodide staining. Representative of three independent experiments. e) Immunoblot of whole cell lysates following overexpression of FLAG-tagged FLCN-WT, FLCN-M222A, or FLCN-F226A in normal HEK293 cells or the ccRCC cell lines 786-O, Caki-1 and Caki-2. f) HEK293 cells were treated with and without 1 μM Rhodamine B-labeled FLCN-10A. Scale bar = 20 μM. g) LDHA activity in Caki-1 cells following 2 h treatment with FLCN-10A peptide.