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. 2001 Apr;67(4):1718–1727. doi: 10.1128/AEM.67.4.1718-1727.2001

FIG. 5.

FIG. 5

IEF patterns obtained under UV light (wavelength, 360 nm) from acetate buffer solutions of siderophores and from filtered GASN culture supernatants. Lanes 1 and 4, supernatants (40 μl) of 3-day GASN cultures of the nonfluorescent strain P. syringae pv. aptata UPB 221 (lane 1) and the fluorescent strain P. syringae pv. aptata LMG 5059 (lane 4); lanes 2 and 3, solutions (60 μl) of purified nonfluorescent siderophore of P. syringae pv. aptata UPB 133 in acetate buffer (pH 5.0) transiently increased to pH 10.0 (lane 2) or not (lane 3).