Figure 4.

Vitamin B₂ promotes the proliferation and migration and inhibits apoptosis of HUVECs. (a) The effect of different concentrations of vitamin B₂ on HUVECs proliferation. (b) The change of proliferation ability of HUVECs in the presence or absence of vitamin B₂ and dexamethasone. (c) Images of the wound healing assay at 72 h showing a larger number of migrated cells in the vitamin B₂ coincubation group than the DEX group. (d) Quantitative analysis of the assay shown in (c). (e) The population of apoptotic cells was measured by Annexin V/PI staining and flow cytometric analysis. (f) Quantitative analysis of the data shown in (e). Each experiment was done in triplicate. ^∗P < 0.05.