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. 2022 Jul 6;2022:4668774. doi: 10.1155/2022/4668774

Figure 4.

Figure 4

HOTAIR upregulates HK2 expression via association with RNA de-methyltransferase FTO. (a) MeRIP assay was used to detect the m6A modification of HK2 mRNA in LC cells. (b) MeRIP assay was used to detect the m6A modification of HK2 mRNA in control and HOTAIR-depleted cells. (c) RIP assay using IgG, FTO, or ALKBH5 antibody was performed in LC cells. The pull-down HOTAIR was then detected using qRT-PCR. (d) The RIP assay using IgG or FTO antibody was performed in control and HOTAIR-depleted LC cells. (e) Overexpression of FTO recued the degradation of HK2 mRNA mediated by HOTAIR knockdown. ∗P < 0.05 compared with the negative control.