Table 6.
In vitro transcription protocol.
| Component | Volume |
|---|---|
| DNA template | 1-2 μg |
| T7 reaction buffer | 5 μl |
| 2′-F NTP mix | 1.5 μl |
| RNA polymerase | 0.5 μl |
| DEPC treated water | Up to 25 μl |
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