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. 2022 Jun 29;607(7918):313–320. doi: 10.1038/s41586-022-04824-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Sampling locations of ancient wolves and one ancient dhole analysed here, on a base map from Natural Earth (naturalearthdata.com). b, Ages and sequencing coverage of ancient wolves. c, PC1 from a PCA on outgroup f₃-statistics plotted against sample age. PCs were calculated from ancient wolves only, with present-day wolves and dogs projected onto the plot. d, Heterozygosity estimates from sampling of two reads at sites ascertained as heterozygous in a coyote. Bars denote 95% CIs from block jackknifing.