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. 2022 Jun 29;607(7918):339–344. doi: 10.1038/s41586-022-04902-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Wild-type Col-0 and 35S::CBP60g plants were syringe-infiltrated with mock (0.25 mM MgCl₂) or Pst DC3000 (10⁶ CFU ml⁻¹) and incubated at 23 °C or 28 °C. a, SA levels in mock- and Pst DC3000-inoculated plants at 24 h (1 dpi). b, Images of leaves from Pst DC3000-inoculated plants at 3 dpi. c, In planta Pst DC3000 bacterial levels at 3 dpi. d,e, In planta Pst DC3000 (avrPphB) (d) or Pst DC3000 (avrRps4) (e) bacterial levels at 3 dpi. f, Heat map of RNA-seq reads for genes that are downregulated in Col-0 grown at 28 °C but fully or partially restored in 35S::CBP60g grown under the same conditions. RPKM, reads per kilobase of transcript per million mapped reads. Data in a,c,e are mean ± s.d. (n = 4 biological replicates) of one representative experiment (out of three independent experiments) analysed by two-way ANOVA with Tukey’s HSD. Results in d are mean ± s.d. (n = 4 biological replicates except 35S::CBP60g at 23 °C (n = 3 biological replicates)) of one representative experiment (out of three independent experiments) analysed by two-way ANOVA with Tukey’s HSD. Exact P-values for all comparisons are shown in the Source Data.

Source data