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. 2022 Jun 29;607(7918):339–344. doi: 10.1038/s41586-022-04902-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a, CBP60g transcript levels in 4-week old 35S::CBP60g at 23 °C or 28 °C 1 day after mock (0.25 mM MgCl₂) treatment or Pst DC3000 infection [1.0 x 10⁶ Colony Forming Units (CFU) mL⁻¹]. b–d, SA levels at 1 dpi (b), symptom expression at 3 dpi (c) and in planta Pst DC3000 [1.0 x 10⁶ Colony Forming Units (CFU) mL⁻¹] bacterial levels at 3 dpi (d) of Col-0 and 35S::CBP60g 16 plants. e–g, bacterial levels in Col-0 and cbp60g-1 plants inoculated with Pst DC3000 (e), Pst DC3000 (avrPphB) (f), and Pst DC3000 (avrRps4) (g) at 3 dpi. h, ICS1 gene expression levels in Pst DC3000 ΔhrcC-infected Col-0 and 35S:CBP60g plants [1.0 x 10⁸ Colony Forming Units (CFU) mL⁻¹] at 12- and 24-h post-inoculation (hpi). i, SA levels in Pst DC3000 ΔhrcC-infected Col-0 and 35S:CBP60g plants (1.0 x 10⁸ Colony Forming Units (CFU) mL⁻¹) at 24 h post-inoculation. j–k, In planta Pst DC3000 (avrPphB) (j), and (avrRps4) (k) bacterial levels of Col-0 and 35S::CBP60g 16 plants at 3 dpi. l, ICS1, EDS1 and PAD4 gene expression levels of Col-0 and 35S::CBP60g plants 1 day after mock (0.25 mM MgCl₂)- and Pst DC3000-infiltration [1.0 x 10⁶ Colony Forming Units (CFU) mL⁻¹]. Results show the means ± S.D. [n = 3 (a, f, g, h) or 4 (b, d, i) biological replicates] from one representative experiment [of two (a, h, i) or three (b, d, f, g) independent experiments] analyzed with two-way ANOVA with Tukey’s HSD for significance. Results in (e) show the means ± S.D. [n = 4 biological replicates except Col-0 at 23 °C (n = 3 biological replicates)] from one representative experiments (of three independent experiments) analyzed with two-way ANOVA with Tukey’s HSD for significance. Results in (j) show the means ± S.D. [n = 4 (Col-0) or 3 (35S::CBP60g 16) biological replicates] from one representative experiments (of three independent experiments) analyzed with two-way ANOVA with Tukey’s HSD for significance. Results in (k) show the means ± S.D. [n = 4 biological replicates except 35S::CBP60g 16 at 23 °C (n = 3 biological replicates)] from one representative experiments (of three independent experiments) analyzed with two-way ANOVA with Tukey’s HSD for significance. Exact P-values for those comparisons that are greater than 0.05 are detailed in the Source Data files

Source data