TABLE 1.
Comparison of the A. aegerita family B DNA polymerases Aa-POLB and Aa-POLB P1 with five related family B DNA polymerases
| Microorganism | Family B DNA polymerase (GenBank accession no.) | Size (aa) | Size (aa) and location of the Pol I to Pol III sequences | % aa similarityb
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Aa-POLB | Aa-POLB P1 | Ss-B1 | Ss-B2 | Ss-B3 | Φ 29 | pEM | ||||
| A. aegerita strain WT-3a | Aa-POLB (AF061244) | 571 | 271 (IRKS–SKEL) | 100 | 96 | 44 | 38 | 40 | 44 | 53 |
| Aa-POLB P1 (AF268233) | 500 | 272 (IRIG–YFYI) | 100 | 41 | 36 | 39 | 45 | 51 | ||
| S. solfataricus strain P2 | Ss-B1 (U92875) | 882 | 327 (RTSA–VNVL) | 100 | 50 | 47 | 40 | 32 | ||
| Ss-B2 (X71597) | 626 | 298 (IMMV–RVYD) | 100 | 44 | 37 | 34 | ||||
| Ss-B3 (Y08257) | 764 | 242 (IRFI–SKKK) | 100 | 35 | 40 | |||||
| Bacteriophage Φ 29 | Replicase (X53370) | 575 | 245 (VRYA–TGTE) | 100 | 47 | |||||
| Agaricus bitorquis plasmid pEM | Replicase (P30322) | 773 | 286 (IRSS–RKPL) | 100 | ||||||
a
The complete sequences of Aa-polB as well as those of Aa-polB P1 in strains WT-3 and WT-11 were identical (see text and reference 4).
b
Based on the amino acid sequences located between the highly conserved Pol I and Pol III domains.